This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa. Highlights: Size range — 10 proteins spanning 10 to kDa Ready-to-use — supplied in a loading buffer for direct loading on gels; no need to boil Sharp bands — color-coded bands of similar intensity for easy visualization Quality tested — each lot evaluated by SDS-PAGE and Western blotting Two reference bands — orange at 70kDa and green at 10kDa Membrane-compatible — colored bands transfer to membranes for Western blotting Includes: Dye-stained proteins in Migration patterns of the PageRuler Prestained Protein Ladder in different electrophoretic conditions. The apparent molecular weight of each protein kDa has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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PageRuler™ vorgefärbte Proteinleiter (10 bis 180 kDa)